Anti-KRT17 Antibody, Rabbit Polyclonal
产品编号:PA00460HuA10
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产品名称:Anti-KRT17 Antibody, Rabbit Polyclonal
经验证的应用:WB/ICC
交叉反应:/
特异性:human KRT17
免疫原:Recombinant human KRT17 protein, fragment Glu84~Leu395; UniprotKB: Q04695
制备方法:Produced in rabbits immunized with human KRT17, and purified by antigen affinity chromatography.
来源:Polyclonal Rabbit IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
状态:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various cell lines using KRT17 antibody (PA00460HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 0.5s.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil sections labelling KRT17 with purified PA00460HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称:PC2, KRT17, K17, PC, PCHC1, Keratin 17, Keratin, type I cytoskeletal 17
背景信息:Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.
全称:Keratin, type I cytoskeletal 17 (KRT17)
说明书:待上传