Anti-KRT17 Antibody, Rabbit Polyclonal

产品编号:PA00460HuA10
   
询价

规格     50uL  100uL  200uL

产品名称Anti-KRT17 Antibody, Rabbit Polyclonal

经验证的应用WB/ICC
 
交叉反应/
 
特异性human KRT17
 
免疫原Recombinant human KRT17 protein, fragment Glu84~Leu395; UniprotKB: Q04695

制备方法Produced in rabbits immunized with human KRT17, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various cell lines  using KRT17 antibody (PA00460HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 0.5s.











 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil sections labelling KRT17 with purified PA00460HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.






别称PC2, KRT17, K17, PC, PCHC1, Keratin 17, Keratin, type I cytoskeletal 17
 
背景信息Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

全称Keratin, type I cytoskeletal 17 (KRT17)

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