Anti-Atp5f1b Antibody, Rabbit Polyclonal

产品编号:PA00499MuA10
   
询价

规格     50uL  100uL  200uL
产品名称Anti-Atp5f1b Antibody, Rabbit Polyclonal

经验证的应用WB/IHC
 
交叉反应Hu/Ra
 
特异性mouse Atp5f1b
 
免疫原Recombinant mouse Atp5f1b protein, fragment Ala47~Ser529; UniprotKB: P56480

制备方法Produced in rabbits immunized with mouse Atp5f1b, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various cell lines  using ATP5B antibody (PA00499MuA10) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution. 




 



Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney sections labelling Atp5f1b with purified PA00499MuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™Epitope Retrieval Solution  (EDTA, pH 9.0) . Tissue was counterstained with Hematoxylin.Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.



别称Atp5b
 
背景信息ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

全称ATP synthase subunit beta (Atp5f1b)

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