Anti-TNFSF13B Antibody, Rabbit Polyclonal
产品编号：PA00516HuA20
询价
规格 50uL 100uL 200uL
产品名称：Anti-TNFSF13B Antibody, Rabbit Polyclonal
经验证的应用：WB
交叉反应：Hu/Mu
特异性：human TNFSF13B
免疫原：Recombinant human TNFSF13B protein, fragment Ala134~Leu285; UniprotKB: Q9Y275
制备方法：Produced in rabbits immunized with human TNFSF13B, and purified by antigen affinity chromatography.
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Western blot analysis of extracts of various cell lines, using ACACA antibody PA00516HuA20 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum sections labelling TNFSF13B with purified PA00516HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
背景信息：BAFF/BLyS/TNFSF13B. B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homo-oligomer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co‑stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14‑16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).
全称：Tumor necrosis factor ligand superfamily member 13B (TNFSF13B)
说明书：
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说明书 |