产品名称:Anti-KRT19 Antibody, Rabbit Polyclonal
经验证的应用:WB
交叉反应:Predict reacts with: Human/Mouse/Rat
特异性:human KRT19
免疫原:Recombinant human KRT19 protein, fragment Tyr127~Asp322; UniprotKB: P08727
制备方法:Produced in rabbits immunized with human KRT19, and purified by antigen affinity chromatography.
来源:Polyclonal Rabbit IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
状态:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure 1. Application in WB
Western blot analysis of extracts of various cell lines and tissues, using KRT19 antibody (PA00104HuA10) at 1μg/mL. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30 s.
别称:CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19
背景信息:Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.
全称:Keratin, type I cytoskeletal 19 (KRT19)