Anti-FCER2 Antibody, Rabbit Polyclonal

产品编号:PA00518HuA20
   
询价

规格     50uL  100uL  200uL




产品名称Anti-FCER2 Antibody, Rabbit Polyclonal
 
经验证的应用:/
 
交叉反应/
 
特异性human FCER2
 
免疫原Recombinant human FCER2 protein, fragment Met150~Ser321; UniprotKB: P06734
 
制备方法Produced in rabbits immunized with human FCER2, and purified by antigen affinity chromatography
 
来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
 
图片:


Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil  sections labelling FCER2 with purified PA00518HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.





别称FceRII, FCER2, BLAST-2, CLEC4J, FCE2, IGEBF, Receptor II For The Fc Region Of Immunoglobulin E, C-type lectin domain family 4 member J, Immunoglobulin E-binding factor, Lymphocyte IgE receptor
 
背景信息
CD23/Fc epsilon RII. CD23 (also named B cell differentiation antigen) is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily (1 - 5). Human CD23 is a 47 kDa, type II transmembrane glycoprotein that is expressed by a wide variety of cell types (6 - 10). The full-length receptor is 321 amino acids (aa) in length and contains a 274 aa extracellular region, a 26 aa transmembrane segment, and a 21 aa cytoplasmic domain. The extracellular region contains a C-type lectin domain and a connecting stalk with coiled-coil topography (3, 11). The lectin domain binds both protein and carbohydrate in an apparently Ca++ independent manner (11). The coiled-coil region contributes to oligomerization (11, 12). The lectin domain in human CD23 (aa 162 - 284) is 64%, 62% and 68% aa identical to the lectin domains in mouse, rat and bovine CD23, respectively. In the cytoplasmic region, two FC isoforms exist which arise from alternate start sites (6, 12). The “a” (or long) isoform begins with the sequence MEEGQYS and is constitutively expressed by B cells. It is believed to participate in IgE-mediated endocytosis (13). The “b” (or short) isoform begins with MNPPSQ and is induced on a wide variety of cell types by IL-4 (6). Fcb reportedly contributes to IgE-mediated phagocytosis (13). Fcb expressing cells include eosinophils, monocytes, visceral smooth muscle and intestinal epithelium (6, 14, 15). At least four soluble forms of CD23 are known to exist. They range in molecular weight from 25 kDa to 37 kDa, with the 25 kDa form predominating in sera (16). Soluble CD23 (sFc) is generated by metalloprotease (ADAM8; ADAM15; ADAM28) and cysteine-protease activity (16 - 18). Cleavage usually occurs between aa 150 - 160 (7, 8). It is unclear if sequential metalloprotease-cysteine protease activity is necessary for the generation of all soluble forms. Both soluble and membrane-bound CD23 show bioactivity. Ligands for CD23 include CD21, IgE, CD11b, and CD11c (19 - 21). CD23 binding to CD11b and Cd11c on monocytes results in oxidative product generation and proinflammatory cytokine release (21). On B cells, sCD23 induces IgE secretion by binding CD21. Conversely, secreted IgE will, in turn, bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (11).
 
全称
Low affinity immunoglobulin epsilon Fc receptor (FCER2)
 
说明书
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