Anti-H3C1 Antibody, Mouse Monoclonal, D3F

产品编号:CM00037HuM10-D3F
   
询价

规格     100ul

说明书

产品名称Anti-H3C1 Antibody, Mouse Monoclonal, D3F
 
克隆号D3F
 
抗体亚型:/
 
经验证的应用/
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原:/
 
制备方法Produced in mouse immunized with H3C1, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various lane7 mouse liver tissue(+++)、lane8 rat heart(+++)、lane9 Jurkat(+++)、lane10 Hela(+++), using H3C1 antibody (CM00037HuM10-D3F) at 2ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1min.







 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin、monkey brain stem、monkey epididymis、monkey kidney、monkey rectum、monkey skeletal muscle、monkey testis、monkey Submandibular gland sections labelling H3C1 with purified CM00037HuM10-D3F at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.



别称Histone H3/a
 
背景信息/

全称Histone H3.1 (H3C1)