产品名称:Anti-H3C1 Antibody, Mouse Monoclonal, D3F
克隆号:D3F
抗体亚型:/
经验证的应用:/
交叉反应:Predict reacts with: Human/Mouse/Rat
特异性:/
免疫原:/
制备方法:Produced in mouse immunized with H3C1, and purified by antigen affinity chromatography.
来源:Monoclonal Mouse IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
浓度:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various lane7 mouse liver tissue(+++)、lane8 rat heart(+++)、lane9 Jurkat(+++)、lane10 Hela(+++), using H3C1 antibody (CM00037HuM10-D3F) at 2ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin、monkey brain stem、monkey epididymis、monkey kidney、monkey rectum、monkey skeletal muscle、monkey testis、monkey Submandibular gland sections labelling H3C1 with purified CM00037HuM10-D3F at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.
别称:Histone H3/a
背景信息:/
全称:Histone H3.1 (H3C1)