产品名称：Anti-ACTA1 Antibody, Mouse Monoclonal, B11T
克隆号：B11T
抗体亚型：/
经验证的应用：WB/IHC
交叉反应：Human/Mouse/Rat
特异性：/
免疫原：A synthetic peptide of human ACTA1
制备方法：Produced in mouse immunized with ACTA1, and purified by antigen affinity chromatography.
来源：Monoclonal Mouse IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
浓度：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Application in WB
Western blot analysis of extracts of various cells lines and tissues, using ACTA1 antibody CM01105HuM10-B11T at 1μg/mL. Secondary antibody: HRP Mouse Anti-Rabbit IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung、 monkey cerebellum、 monkey skeletal muscle、 monkey jejunum、 monkey kidney、 monkey liver、 monkey lung、 monkey rectum、 monkey salivary gland、 monkey submandibular gland sections labelling ACTA1 with purified CM01105HuM10-B11T at 5μg/mL dilution. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：ACTA, Alpha-actin-1, Actin, alpha skeletal muscle, intermediate form
背景信息：Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
全称：Actin, alpha skeletal muscle (ACTA1)