产品名称:Anti-GSK3B Antibody, Mouse Monoclonal, A6T
克隆号:A6T
抗体亚型:/
经验证的应用:/
交叉反应:Predict reacts with: Human/Mouse/Rat
特异性:/
免疫原:A synthetic peptide of human GSK3B
制备方法:Produced in mouse immunized with GSK3B, and purified by antigen affinity chromatography.
来源:Monoclonal Mouse IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
浓度:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various lane1 Hela(+++)、lane2 NIH-3T3(+++)、lane3 rat brain(+++), using GSK3B antibody (MA00113HuM10-A6T) at 5ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 2.5min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Liver cancer sections labelling GSK3B with purified MA00113HuM10-A6T at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.
背景信息:Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.
全称:Glycogen synthase kinase-3 beta (GSK3B)