产品名称：Anti-MAP1LC3A Antibody, Mouse Monoclonal, B1F
克隆号：B1F
抗体亚型：/
经验证的应用：/
交叉反应：Predict reacts with: Human/Mouse/Rat
特异性：/
免疫原：/
制备方法：Produced in mouse immunized with MAP1LC3A, and purified by antigen affinity chromatography.
来源：Monoclonal Mouse IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
浓度：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Western blot analysis of extracts of various lane1 mouse brain(+++)、lane2 Rat brain(+++), using MAP1LC3A antibody (MA01074HuM10-B1F) at 5ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain sections labelling MAP1LC3A with purified MA01074HuM10-B1F at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.
背景信息：LC3A. Microtubule-associated proteins 1A/1B light chain 3A (also known as MAP1LC3A) is an Ubiquitin-like modifier of the ATG8 family and is involved in the formation of the autophagosomes. This protein interacts directly with Sequestosome-1 and is recruited to inclusion bodies containing polyubiquitinated protein aggregates where it plays a role in inclusion body degradation by autophagy. MAP1LC3A-Rhodamine110 (Rh110) is a fluorogenic substrate for the autophagy hydrolase ATG4B. This substrate is useful for studying ATG4B when detection sensitivity or continuous monitoring of activity is essential.
全称：Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A)